Please use this identifier to cite or link to this item: http://ena.lp.edu.ua:8080/handle/ntb/19815
Title: Enzymatic synthesis of (R)-mandelonitrile by using cross linked enzyme aggregates of Prunus cerasifera hydroxynitrile lyase
Authors: Dilek Alagöz
Yıldırım Deniz
Tükel S. Seyhan
Bibliographic description (Ukraine): Alagöz D. Enzymatic synthesis of (R)-mandelonitrile by using cross linked enzyme aggregates of Prunus cerasifera hydroxynitrile lyase / Dilek Alagöz, Deniz Yıldırım, S. Seyhan Tükel // Хімія та хімічні технології : матеріали ІI Міжнародної конференції молодих вчених CCT-2011, 24–26 листопада 2011 року, Україна, Львів / Національний університет "Львівська політехніка". – Львів : Видавництво Львівської політехніки, 2011. – С. 136–137. – (3-й Міжнародний молодіжний фестиваль науки "Litteris et Artibus"). – Bibliography: 14 titles.
Issue Date: 2011
Publisher: Видавництво Львівської політехніки
Keywords: hydroxynitrile lyase
cross linked enzyme aggregates
mandelonitrile
enantiopurity
immobilization
Abstract: In this study, cross-linked enzyme aggregates (CLEA) of Prunus cerasifera hydroxynitrile lyase (PcHNL-CLEA) were prepared from seeds of Prunus cerasifera and characterized for the first time. The influence of pH and temperature on the PcHNL-CLEA lyase activity was investigated. The optimal pH and temperature for lyase activity of PcHNL-CLEA were 5.5 and 25°C, respectively. We determined Km and Vmax of PcHNL-CLEA as 4.0 mM and 22 U/g PcHNL-CLEA, respectively. The thermal stability of PcHNL-CLEA at 25 and 50°C was studied. PcHNL-CLEA retained 72% of its initial activity when incubated at 25°C and 54% of initial activity when stored at 50°C at the end of 16 hours. The PcHNLCLEA was also used for the enantiopure mandelonitrile synthesis from benzaldehyde and potassium cyanide. The yield and enantiopurity of (R)-mandelonitrile were obtained as 100 and 94% respectively.
URI: http://ena.lp.edu.ua:8080/handle/ntb/19815
Content type: Article
Appears in Collections:Хімія та хімічні технології (CCT-2011). – 2011 р.

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